batch purification造句
例句與造句
- Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation . 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies . 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs , prepare a 50 % slurry for batch purification method , pack column with matrix slurry
三、 gst一hbrp重組蛋白的純化1 .超聲破碎細(xì)胞,離心,上清和沉淀進(jìn)行sds一page電泳分析2 .樣品處理提取包涵體,變性后,加人用pbs平衡過(guò)的以u(píng)tal腸onesepb抓脫4b ,室溫下孵育3 .以u(píng)tadtionesepharose4b柱純化以u(píng)tad云onesepharose4b柱的準(zhǔn)備根據(jù)毛山lel ,決定純化所需的gll衛(wèi)ta1如oneseph娜se4b的柱床體積,用預(yù)冷的1xpbs清洗cldtathionese戶, se4b ,得到50 %的基質(zhì) - It's difficult to find batch purification in a sentence. 用batch purification造句挺難的